Therefore, the main objective of this study was to measure how bioreactors influence microbial concentrations and AMR concentrations in tile drainage water with different flow conditions and microbial communities.Ī column study was conducted to simulate an existing, field denitrifying woodchip bioreactor.įour synthetic waters were spiked with different combinations of microbes isolated from the influent of a field-scale bioreactor and pumped through the columns during the experiment. The effect of woodchip bioreactors on microbial concentration in subsurface drainage water and the associated risk of antibiotic resistance dissemination Article Full-text available Feb 2020 Sara Mardani Rachel McDaniel Bruce H.īleakley Louis Amegbletor Woodchip bioreactors (WBs) are a promising technology capable of decreasing nitrate concentration in subsurface (tile) drainage system (SDS) effluent.Īlthough they are effective in removing nitrate, little is known regarding the potential impact on microbes and antimicrobial resistance (AMR) in subsurface drainage waters.
Since OD is directly proportional to the cell concentration (Dubey, 2014 Madigan et al., 2010), the concentration of each culture was estimated using the OD 600 and the concentration-OD 600 relationship for each indigenous microbe determined using methods discussed in section 2.4.Īfterward, around 0.1 mL of each culture was immediately added to 20 mL of phosphate buffered saline solution (PBS) and diluted to achieve the desired concentration of approximately 2 10 1 CFUmL of each microbe to reach a total concentration of 2 10 2 CFUmL before being added to the inoculation solution.
The OD 600 of the resulting broth culture was measured and used to estimate the concentration of each culture.
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